The Election and Coronation Night of Miss Colombia 1999-2000 took place in Cartagena de Indias, Colombia, on 1999-11-14 at the Julio César Turbay Convention Center, marking the 47th edition. The winner was Miss Cundinamarca, Catalina Inés Acosta Albarracín, who was crowned by her predecessor Marianella Maal Pacini.