Señorita Colombia 2000–2001, the 48th edition of the Concurso Nacional de Belleza de Colombia, took place on 12 November 2000 at the Centro de Convenciones in Cartagena de Indias. Andrea María Noceti Gómez (Señorita Cartagena D.T. y C.) won the title, crowned by outgoing queen Catalina Inés Acosta Albarracín. Twenty-two candidates competed. The show featured performances by Cabas, Juanes, and Franco de Vita and was broadcast by RCN Televisión.